Anti-VCIP 135 antibody (ab97812)
Key features and details
- Rabbit polyclonal to VCIP 135
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VCIP 135 antibody -
Description
Rabbit polyclonal to VCIP 135 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 955 and 1217 of VCIP 135 (Q96JH7)
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Positive control
- H1299 whole cell lysate; 293T, A431, HeLaS3, HepG2, MOLT4 or Raji lysates. SW480 xenograft (IHC-P)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 134 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 134 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. -
Sequence similarities
Contains 1 OTU domain. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus, Golgi stack. Associated with Golgi stacks and endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 80124 Human
- Entrez Gene: 70675 Mouse
- Entrez Gene: 286761 Rat
- Omim: 611745 Human
- SwissProt: Q96JH7 Human
- SwissProt: Q8CDG3 Mouse
- SwissProt: Q8CF97 Rat
- Unigene: 632066 Human
see all -
Alternative names
- Deubiquitinating protein VCIP135 antibody
- DUBA3 antibody
- KIAA1850 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab97812 has not yet been referenced specifically in any publications.