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Storage instructions Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000. Predicted molecular weight: 134 kDa.
Use at an assay dependent concentration.
Function Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.
Sequence similarities Contains 1 OTU domain.
Cellular localization Endoplasmic reticulum. Golgi apparatus, Golgi stack. Associated with Golgi stacks and endoplasmic reticulum.
Information by UniProt
Deubiquitinating protein VCIP135 antibody
Anti-VCIP 135 antibody images
Western blot - VCIP 135 antibody (ab97812)
Anti-VCIP 135 antibody (ab97812) at 1/5000 dilution + H1299 whole cells lysate at 30 µg Predicted band size : 134 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VCIP 135 antibody (ab97812)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ab97812 at 1/100 dilution.
References for Anti-VCIP 135 antibody (ab97812)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"