VCP antibody [5] (ab11433)

Product Name 

VCP antibody [5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [5] to VCP

Immunogen 

Synthetic peptide: CGGSVYTEDNDDDLYG , corresponding to amino acids 792-806 of Human VCP (with added N-terninal Cysteine).
(Peptide available as ab39788.)
BLAST 'CGGSVYTEDNDDDLYG' with or

Reacts with 

Hu, Ms, Rat, Cow

Predicted to react 
(species key)

Pig
(Due to sequence homology)

Tested applications 
(see key)

IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/500.
IHC-Fr: 1/500.
IP: Use at an assay dependent dilution.
WB: 1/2000. Detects a band of approximately 97 kDa. Can be blocked with VCP peptide (792-806) (ab39788).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysate from cultured human B cells.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Signal Transduction >> Protein Trafficking >> ER Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Synuclein

Relevance 

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

5

Isotype 

IgG2a

Purity 

Ascites

Storage buffer 

Preservative: 0.05% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for VCP antibody [5] images, references, products related to ab11433 and other tools.

VCP antibody [5] images:

References for VCP antibody [5] (ab11433)

  This product has been used in: (two most recent references)

Deng X et al. Exploring the priming mechanism of liver regeneration: proteins and protein complexes. Proteomics 9:2202-16 (2009). GSA. PubMed: 19322782

Humphreys D et al. The Salmonella effector SptP dephosphorylates host AAA+ ATPase VCP to promote development of its intracellular replicative niche. Cell Host Microbe 5:225-33 (2009). WB; Human, Pig. PubMed: 19286132

See all 9 publication references for this product.


If you publish research using ab11433 please let us know so that we can cite the reference on this datasheet.

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