Overview

  • Product nameAnti-VCP antibody [EPR3307(2)]
    See all VCP primary antibodies
  • Description
    Rabbit monoclonal [EPR3307(2)] to VCP
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human VCP

  • Positive control
    • MCF-7 cell lysate, HeLa cell lysate, A549 cell lysate; SH-SY5Y cell lysate; human breast carcinoma tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody. 

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 89 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • FunctionNecessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
  • Involvement in diseaseDefects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
  • Sequence similaritiesBelongs to the AAA ATPase family.
  • Post-translational
    modifications
    Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15S Mg(2+) ATPase p97 subunit antibody
    • 15S Mg(2+)-ATPase p97 subunit antibody
    • ALS14 antibody
    • ATPase p97 antibody
    • CDC48 antibody
    • IBMPFD antibody
    • MGC131997 antibody
    • MGC148092 antibody
    • MGC8560 antibody
    • p97 antibody
    • TER ATPase antibody
    • TERA antibody
    • TERA_HUMAN antibody
    • Transitional endoplasmic reticulum ATPase antibody
    • Valosin containing protein antibody
    • Valosin-containing protein antibody
    • VCP antibody
    • Yeast Cdc48p homolog antibody
    see all

Anti-VCP antibody [EPR3307(2)] images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab109240 at 1/100 dilution.
  • Immunofluorescent staining of HeLa cells using ab109240 at 1/100 dilution.
  • All lanes : Anti-VCP antibody [EPR3307(2)] (ab109240) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg/ml per lane.


    Predicted band size : 89 kDa
  • Overlay histogram showing HL60 cells stained with ab109240 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109240, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-VCP antibody [EPR3307(2)] (ab109240)

This product has been referenced in:
  • Guo X  et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun 7:12646 (2016). Read more (PubMed: 27561680) »
  • Zhang Y  et al. Effects of 1.8 GHz radiofrequency radiation on protein expression in human lens epithelial cells. Hum Exp Toxicol N/A:N/A (2013). WB ; Human . Read more (PubMed: 23338683) »

See all 4 Publications for this product

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