Tissue/ cell preparation (Human). Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Our Abpromise guarantee covers the use of ab3983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 µg/ml. Staining technique: Membrane permeabilization (acetone) is essential. ab3983 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 220 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
ab3983 at 10ug/ml staining 220kDa intra acrosomal protein in human sperms by ICC/IF. Cells were permeabilized with acetone.