Overview

  • Product nameAnti-VCP antibody [Hs-14] (FITC)
    See all VCP primary antibodies
  • Description
    Mouse monoclonal [Hs-14] to VCP (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human VCP. Freshly ejaculated human sperm washed in PBS & extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and then used for immunization.

  • Positive control
    • Sperm cells.
  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab77238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 20µl for 100µl cell suspension.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNecessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
    • Involvement in diseaseDefects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
    • Sequence similaritiesBelongs to the AAA ATPase family.
    • Post-translational
      modifications
      Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • Cellular localizationCytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acrosomal protein SP 10 antibody
      • Acrosomal protein SP-10 antibody
      • Acrosomal vesicle protein 1 antibody
      • ACRV1 antibody
      • ASPX_HUMAN antibody
      • D11S4365 antibody
      • Msa63 antibody
      • SP 10 antibody
      • Sp10 antibody
      • SPACA2 antibody
      • Sperm protein 10 antibody
      see all

    References for Anti-VCP antibody [Hs-14] (FITC) (ab77238)

    This product has been referenced in:
    • Peknicova J  et al. Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol 53:42-9 (2005). Read more (PubMed: 15667524) »
    • Chládek D  et al. [Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization] Ceska Gynekol 65:28-32 (2000). Read more (PubMed: 10750294) »

    See all 2 Publications for this product

    Product Wall

    The concentration is 190 ug/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"