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Western blot - VDAC/Porin antibody (ab28777)
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Immunohistochemistry (Paraffin-embedded sections) - VDAC/Porin antibody (ab28777)
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Product Name
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VDAC/Porin antibody
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See all VDAC/Porin antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VDAC/Porin
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Immunogen
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A region within synthetic peptide: SAKVNNSSLI GLGYTQTLKP GIKLTLSALL DGKNVNAGGH KLGLGLEFQA, corresponding to C terminal amino acids 234-283 of Human VDAC/Porin
BLAST 'SAKVNNSSLI GLGYTQTLKP GIKLTLSALL DGKNVNAGGH KLGLGLEFQA ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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ab28777 recognises Outer mitochondrial membrane protein porin (VDAC1).
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/312500.
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Predicted molecular weight: 31 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate. Human liver tissue
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Cellular localization
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Mitochondrial outer membrane and plasma membrane.
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Research areas
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Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Mitochondrial
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Voltage dependent anion selective channel protein 1 (VDAC/Porin) belongs to the eukaryotic mitochondrial porin family and forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation selective. VDAC/Porin expression is observed in the heart,liver and skeletal muscle.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC/Porin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC/Porin antibody images, references, products related to ab28777 and other tools.
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VDAC/Porin antibody images:
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 Western blot - VDAC/Porin antibody (ab28777)
VDAC/Porin antibody (ab28777) at 1.25 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 31 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
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Immunohistochemistry (Paraffin-embedded sections) - VDAC/Porin antibody (ab28777)
ab28777, at a concentration of 6.0µg/ml, staining human VDAC1 in (liver) by Immunohistochemistry, Paraffin embedded tissue. Arrows indicate positively labelled cells (hepatocytes). Magnification 400X.
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Search PubMed (MEDLINE) for references to VDAC/Porin
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VDAC/Porin antibody - more information
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Customer reviews (feedback) regarding VDAC/Porin antibody |
Customer FAQs regarding VDAC/Porin antibody |
Protocols for VDAC/Porin antibody |
Price and availability of products related to VDAC/Porin antibody |
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VDAC/Porin antibody - related products:
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VDAC/Porin antibody - other tools:
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Contact Abcam with a Technical Enquiry about VDAC/Porin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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