Recombinant
RabMAb

Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

Overview

  • Product name
    Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control
    See all VDAC1 / Porin primary antibodies
  • Description
    Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human VDAC1/ Porin aa 1-100 (N terminal).
    Database link: P21796

  • Positive control
    • WB: HepG2, Jurkat, 293T, HeLa cell lysates IHC-P: Human liver and heart tissues
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity
    Heart, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the eukaryotic mitochondrial porin family.
  • Domain
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization
    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hVDAC1 antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • Porin antibody
    • VDAC 1 antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage Dependent Anion Channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution (purified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HEK293 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution (purified)

    Lane 1 : mouse kidney lysate
    Lane 2 : rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/2000 dilution (purified) + Jurkat cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/5000 dilution (unpurified) + Rat cerebellum whole tissue lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) (undiluted)
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa


    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 31 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded human normal kidney tissue using unpurified ab154856 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using unpurified ab154856 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded human thyroid gland carcinoma tissue using unpurified ab154856 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab154856 showing +ve staining.

References

This product has been referenced in:
  • Tegelberg S  et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis 12:73 (2017). Read more (PubMed: 28427446) »
  • Tang C  et al. Aspartate ß-hydroxylase disrupts mitochondrial DNA stability and function in hepatocellular carcinoma. Oncogenesis 6:e362 (2017). Read more (PubMed: 28714949) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Polina Vishnyakova

Verified customer

Submitted Oct 01 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Rat Tissue lysate - whole (cerebellum)
Specification
cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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