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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-VDAC1/Porin antibody (HRP)
Description
Rabbit polyclonal to VDAC1/Porin (HRP)
Conjugation
HRP
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Sheep, Rabbit, Chicken, Cow, Pig, Zebrafish, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human VDAC1/Porin - HRP .
(Peptide available as ab16131.)
Positive control
This antibody gave a positive signal in the following lysates: Caco-2 Whole Cell HepG2 Whole Cell Rat Kidney Tissue HeLa Whole Cell SW480 Whole Cell Mouse Skeletal Muscle Tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml
WB: 1/5000Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Target
Relevance
The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin) is an outer membrane mitochondrial protein. The VDAC protein is thought to form the major pores through which adenine nucleotides cross the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT) and cyclophilin D (CypD) is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.
Cellular localization
Outer membranes of all eukaryotic mitochondria
Alternative names
- hVDAC1 antibody
- MGC111064 antibody
- Mithocondrial porin antibody
see all
Database links
- Entrez Gene: 416320 Chicken
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
see all
Anti-VDAC1/Porin antibody (HRP) images:
Western blot - VDAC1/Porin - HRP antibody (HRP) (ab40747)
All lanes : Anti-VDAC1/Porin antibody (HRP) (ab40747) at 1/5000 dilution
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Rat Kidney Tissue Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
Lane 6 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin - HRP antibody (HRP) (ab40747)
IHC image of ab40747 staining VDAC1/Porin in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40747, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-VDAC1/Porin antibody (HRP) (ab40747)
This product has been referenced in:
- Hall MEet al. Systolic dysfunction in cardiac-specific ligand-inducible MerCreMer transgenic mice. Am J Physiol Heart Circ Physiol 301:H253-60 (2011). WB; Mouse.Read more (PubMed: 21536850) »
See 1 publication for this product
Publishing research using ab40747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"