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Product name
Anti-VDAC1/Porin antibody (HRP)
Description
Rabbit polyclonal to VDAC1/Porin (HRP)
Conjugation
HRP
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Sheep, Rabbit, Chicken, Cow, Pig, Zebrafish, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human VDAC1/Porin - HRP .
(Peptide available as ab161 31.)
Positive control
This antibody gave a positive signal in the following lysates: Caco-2 Whole Cell HepG2 Whole Cell Rat Kidney Tissue HeLa Whole Cell SW480 Whole Cell Mouse Skeletal Muscle Tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Isotype/Loading Controls >> Loading Controls >> VDAC1 - Mitochondrial Preps
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml.
WB: 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Target
Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificity
Heart, liver and skeletal muscle.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localization
Mitochondrion outer membrane. Cell membrane.
Target information above from: UniProt accessionP21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hVDAC1 antibody
- MGC111064 antibody
- Mithocondrial porin antibody
see all
Database links
- Entrez Gene: 416320 Chicken
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
see all
Anti-VDAC1/Porin antibody (HRP) images:
Western blot - VDAC1/Porin - HRP antibody (HRP) (ab40747)
All lanes : Anti-VDAC1/Porin antibody (HRP) (ab40747) at 1/5000 dilution
Lane 1 :
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Rat Kidney Tissue Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 :
Lane 6 :
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin - HRP antibody (HRP) (ab40747)
IHC image of ab40747 staining VDAC1/Porin in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40747, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-VDAC1/Porin antibody (HRP) (ab40747)
This product has been referenced in:
- Hall ME et al. Systolic dysfunction in cardiac-specific ligand-inducible MerCreMer transgenic mice. Am J Physiol Heart Circ Physiol 301:H253-60 (2011). WB; Mouse.Read more (PubMed: 21536850) »
See 1 publication for this product
Publishing research using ab40747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"