Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)

Overview

  • Product name
    Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
    See all VDAC1/Porin primary antibodies
  • Description
    Mouse monoclonal [16G9E6BC4] to VDAC1/Porin - Mitochondrial Loading Control
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified) corresponding to Saccharomyces cerevisiae VDAC1/Porin.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • Function
    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity
    Heart, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the eukaryotic mitochondrial porin family.
  • Domain
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization
    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • N2441 antibody
      • OMP2 antibody
      • POR1 antibody
      • hVDAC1 antibody
      • MGC111064 antibody
      • Mitochondrial Porin antibody
      • Outer mitochondrial membrane protein porin 1 antibody
      • Plasmalemmal porin antibody
      • Porin 31HL antibody
      • Porin 31HM antibody
      • VDAC antibody
      • VDAC-1 antibody
      • Vdac1 antibody
      • VDAC1_HUMAN antibody
      • Voltage dependent anion channel 1 antibody
      • Voltage dependent anion selective channel protein 1 antibody
      • Voltage-dependent anion-selective channel protein 1 antibody
      • YNL055C antibody
      • YNL2441C antibody
      see all

    Images

    • Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.

      Predicted band size : 30 kDa

    References

    This product has been referenced in:
    • Ying G  et al. Small GTPases Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors. PLoS One 11:e0161236 (2016). Read more (PubMed: 27529348) »
    • Soloveychik M  et al. Mitochondrial control through nutritionally regulated global histone H3 lysine-4 demethylation. Sci Rep 6:37942 (2016). WB ; Saccharomyces cerevisiae . Read more (PubMed: 27897198) »

    See all 17 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Username

    Prof. Chichung Lie

    Verified customer

    Submitted Aug 01 2013

    Thank you for your enquiry.
    Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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