- Datasheet
- Specific References (23)
- Protocols
Overview
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Product nameAnti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies -
DescriptionRabbit polyclonal to VDAC1/Porin
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Host speciesRabbit
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SpecificityCross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial).
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Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Cow, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human VDAC1/Porin aa 185-197.
Sequence:NDGTEFGGSIYQK
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Positive control
- WB: rat heart whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was affinity purified from monospecific antiserum.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab34726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/10000 - 1/50000. | |
| WB | 1/500 - 1/3000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. Fixation with PFA requires a denaturing step, see Abreview by M.Schrader. |
Target
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FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
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Tissue specificityHeart, liver and skeletal muscle.
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Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
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DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
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Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 100008751 Rabbit
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
see all -
Alternative names
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody (ab34726)IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody (ab34726)Image courtesy of M Schrader by Abreview.ab34726 at a 1/100 dilution staining VDAC1/Porin in COS7 cells by Immunocytochemistry/ Immunofluorescence.The antibody does not work with a standard fixation for ICC/IF as it does not recognize the non-denatured protein. However, applying a fixation for hidden antigens (modified from Peränen J, Rikkonen M, Kääriäinen L. A method for exposing hidden antigenic sites in paraformaldehyde-fixed cultured cells, applied to initially unreactive antibodies. J Histochem Cytochem. 1993 Mar41(3):447-54) results in very good recognition of VDAC/porin. Denaturing is achieved by Guanidine hydrochloride in 50 mM Tris-HCL pH 7.5 after fixation (3% pFA, 0.05% GA) and permeabilization (0.2% TX-100). The secondary used was a TRITC conjugated donkey anti-rabbit used at a 1/100 dilution.
Protocols
References
This product has been referenced in:
- Kazak L et al. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Proc Natl Acad Sci U S A 114:7981-7986 (2017). WB ; Mouse . Read more (PubMed: 28630339) »
- Chen Z et al. Hyperoside alleviates adriamycin-induced podocyte injury via inhibiting mitochondrial fission. Oncotarget 8:88792-88803 (2017). Read more (PubMed: 29179476) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"