Overview

  • Product name
  • Description
    Rabbit polyclonal to VDAC1/Porin
  • Specificity
    Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial).
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human VDAC1/Porin aa 185-197.
    Sequence:

    NDGTEFGGSIYQK

  • Positive control
    • WB: rat heart whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab34726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Fixation with PFA requires a denaturing step, see Abreview by M.Schrader.

Target

  • Function
    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity
    Heart, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the eukaryotic mitochondrial porin family.
  • Domain
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization
    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Images

  • IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab34726 at a 1/100 dilution staining VDAC1/Porin in COS7 cells by Immunocytochemistry/ Immunofluorescence.The antibody does not work with a standard fixation for ICC/IF as it does not recognize the non-denatured protein. However, applying a fixation for hidden antigens (modified from Peränen J, Rikkonen M, Kääriäinen L. A method for exposing hidden antigenic sites in paraformaldehyde-fixed cultured cells, applied to initially unreactive antibodies. J Histochem Cytochem. 1993 Mar41(3):447-54) results in very good recognition of VDAC/porin. Denaturing is achieved by Guanidine hydrochloride in 50 mM Tris-HCL pH 7.5 after fixation (3% pFA, 0.05% GA) and permeabilization (0.2% TX-100). The secondary used was a TRITC conjugated donkey anti-rabbit used at a 1/100 dilution.

References

This product has been referenced in:
  • Kazak L  et al. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Proc Natl Acad Sci U S A 114:7981-7986 (2017). WB ; Mouse . Read more (PubMed: 28630339) »
  • Hou S  et al. Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury. Int J Mol Sci 17:N/A (2016). WB ; Rat . Read more (PubMed: 27455249) »

See all 22 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Ictidomys tridecemlineatus Tissue lysate - other (Liver Mitochondria)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Liver Mitochondria
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Amanda Maccannell

Verified customer

Submitted Dec 02 2016

Thank you for contacting us again.

I have issued a free of charge replacement with a different lot number, but it looks like that this vial was already on the pick list for another order. Therefore, the order is waiting to receive a vial we al...

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Thank you for the clarification!


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of another batchwith the order numbe...

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Thank you for contacting us.

Although other lots of this antibody have worked well under these former conditions, all individual antibodies will require individual optimization.

According to the datasheet, 1/500 is within the recom...

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Thank you for your reply. For broad reactivity, I would recommend our ab34726. This antibody is known to react with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). I hope this helps, please let me know if you need any addi...

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Thank you for your enquiry.
Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer...

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Thank you for your inquiry. VDAC1 is a mitochondrial protein and I believe its level of expression is pretty ubiquitous in all tissues, cancerous and normal. According to the Human Protein Atlas, VDAC1 is expressed in most normal and cancer tis...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer-associated fibroblasts of prostate carcinom)
Loading amount
20 µg
Specification
cancer-associated fibroblasts of prostate carcinom
Gel Running Conditions
Reduced Denaturing (Invitrogen's NuPAGE 4-12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Alexander Henke

Verified customer

Submitted Aug 03 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount
30 µg
Specification
protein lysate from lymph node
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Angela Moreno

Verified customer

Submitted Mar 04 2011

Thank you for your inquiry. Cells should be permeabilized with Triton X-100 and then incubated in 6 N Guanidine HCl in 50mM Tris-HCl, pH 7.5 for 10 minutes at room temperature. I hope this information is helpful to you. Please do not hesitate ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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