Anti-VDAC1/Porin antibody (ab34726)
Key features and details
- Rabbit polyclonal to VDAC1/Porin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies -
Description
Rabbit polyclonal to VDAC1/Porin -
Host species
Rabbit -
Specificity
Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow, Pig, Zebrafish -
Immunogen
Synthetic peptide corresponding to Human VDAC1/Porin aa 185-197.
Sequence:NDGTEFGGSIYQK
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from monospecific antiserum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
Tissue specificity
Heart, liver and skeletal muscle. -
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family. -
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
Cellular localization
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 100008751 Rabbit
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
- SwissProt: P45879 Cow
see all -
Alternative names
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
Images
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IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (59)
ab34726 has been referenced in 59 publications.
- Pinho SA et al. Mitochondrial and metabolic remodelling in human skin fibroblasts in response to glucose availability. FEBS J 289:5198-5217 (2022). PubMed: 35213938
- Sawicka E et al. The Influence of Interaction between Cadmium with 17β-Estradiol, 2-Methoxyestradiol and 16α-Hydroxyestrone on Viability and p-Glycoprotein in Ovarian Cancer Cell Line. Int J Mol Sci 23:N/A (2022). PubMed: 35269769
- Wang Q et al. Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy. Ann Clin Transl Neurol 9:1813-1819 (2022). PubMed: 36151849
- Wastag M et al. Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro. Saudi Pharm J 30:1387-1395 (2022). PubMed: 36387339
- Latina V et al. Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease. Acta Neuropathol Commun 9:38 (2021). PubMed: 33750467