Overview

  • Product nameAnti-VDAC1/Porin antibody
    See all VDAC1/Porin primary antibodies
  • Description
    Mouse monoclonal to VDAC1/Porin
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human VDAC1/Porin aa 156-276.
    Sequence:

    NFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETA VNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGI KLTLSALLDGKNVNAGGHKLG


    Database link: P21796

  • Positive control
    • immunogen

Properties

Applications

Our Abpromise guarantee covers the use of ab76441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 31 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
    • Tissue specificityHeart, liver and skeletal muscle.
    • Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
    • DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
    • Cellular localizationMitochondrion outer membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • N2441 antibody
      • OMP2 antibody
      • POR1 antibody
      • hVDAC1 antibody
      • MGC111064 antibody
      • Mitochondrial Porin antibody
      • Outer mitochondrial membrane protein porin 1 antibody
      • Plasmalemmal porin antibody
      • Porin 31HL antibody
      • Porin 31HM antibody
      • VDAC antibody
      • VDAC-1 antibody
      • Vdac1 antibody
      • VDAC1_HUMAN antibody
      • Voltage dependent anion channel 1 antibody
      • Voltage dependent anion selective channel protein 1 antibody
      • Voltage-dependent anion-selective channel protein 1 antibody
      • YNL055C antibody
      • YNL2441C antibody
      see all

    Anti-VDAC1/Porin antibody images

    • Anti-VDAC1/Porin antibody (ab76441) at 1/500 dilution + immunogen at 0.1 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

      Predicted band size : 31 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

    References for Anti-VDAC1/Porin antibody (ab76441)

    ab76441 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76441.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"