Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (HRP) (ab40747)

Overview

  • Product nameAnti-VDAC1/Porin antibody - Mitochondrial Loading Control (HRP)
    See all VDAC1/Porin primary antibodies
  • Description
    Rabbit polyclonal to VDAC1/Porin - Mitochondrial Loading Control (HRP)
  • ConjugationHRP
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Chicken, Cow, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human VDAC1/Porin aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human VDAC1/Porin - HRP .

    (Peptide available as ab16131.)
    (Peptide available as ab16131, ab75649)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Caco-2 Whole Cell HepG2 Whole Cell Rat Kidney Tissue HeLa Whole Cell SW480 Whole Cell Mouse Skeletal Muscle Tissue IHC-P: normal human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab40747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human VDAC1 / Porin peptide (ab16131).

Target

  • FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificityHeart, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
  • DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localizationMitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mithocondrial porin antibody
    • Mitochondrial Porin antibody
    • OPM2, yeast, human complement of antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31 HL antibody
    • Porin 31 HM antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • Porin antibody
    • PORIN-31-HL antibody
    • VDAC 1 antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (HRP) images

  • All lanes : Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (HRP) (ab40747) at 1/5000 dilution

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Rat Kidney Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
    Lane 6 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab40747 staining VDAC1/Porin in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40747, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (HRP) (ab40747)

This product has been referenced in:
  • Sakellariou G  et al. Studies of mitochondrial and non-mitochondrial sources implicate NADPH oxidase(s) in the increased skeletal muscle superoxide generation that occurs during contractile activity. Antioxid Redox Signal : (2012). WB ; Mouse . Read more (PubMed: 23050834) »
  • Hall ME  et al. Systolic dysfunction in cardiac-specific ligand-inducible MerCreMer transgenic mice. Am J Physiol Heart Circ Physiol 301:H253-60 (2011). WB ; Mouse . Read more (PubMed: 21536850) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Skeletal muscle)
Loading amount 20 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 lung adenocarcinoma)
Loading amount 50 µg
Specification A549 lung adenocarcinoma
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human cancer cells)
Loading amount 200000 cells
Specification Human cancer cells
Gel Running Conditions Reduced Denaturing (10% SDS PAGE gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Vincent Denis

Verified customer

Submitted Nov 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"