Overview

  • Product name
  • Description
    Rabbit polyclonal to VDAC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human VDAC2.

  • Positive control
    • This antibody gave a positive signal in Human Brain tissue lysate as well as both HeLa and HepG2 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

Target

  • Function
    Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
  • Tissue specificity
    Expressed in all tissues examined.
  • Sequence similarities
    Belongs to the eukaryotic mitochondrial porin family.
  • Domain
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-36 vdac antibody
    • FLJ23841 antibody
    • hVDAC2 antibody
    • OTTHUMP00000019878 antibody
    • OTTHUMP00000019879 antibody
    • OTTHUMP00000019880 antibody
    • Outer mitochondrial membrane protein porin 2 antibody
    • POR antibody
    • Porin-2 antibody
    • VDAC 2 antibody
    • VDAC-2 antibody
    • VDAC2 antibody
    • VDAC2_HUMAN antibody
    • Vdac6 antibody
    • Voltage dependent anion channel 2 antibody
    • Voltage dependent anion selective channel protein 2 antibody
    • Voltage-dependent anion-selective channel protein 2 antibody
    see all

Images

  • All lanes : Anti-VDAC2 antibody (ab100956) at 1 µg/ml (Milk Blocked - 3%)

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 16 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The predicted molecular weight of VDAC2 is 31 kDa (SwissProt), however we expect to observe a banding pattern around 35 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab100956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab100956.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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