1/100 - 1/500. Perform heat mediated antigen retrieval with
10mM Citrate buffer (PH6.0) or Tris-EDTA buffer ( PH8.0)).
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Expressed in all tissues examined.
Belongs to the eukaryotic mitochondrial porin family.
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.