Overview

  • Product name
  • Description
    Goat polyclonal to VDAC2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-GHKVGLALELEA

    , corresponding to C terminal amino acids 283-294 of Human VDAC2

  • Positive control
    • Human Brain and Testes lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab37985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
  • Tissue specificity
    Expressed in all tissues examined.
  • Sequence similarities
    Belongs to the eukaryotic mitochondrial porin family.
  • Domain
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B-36 vdac antibody
    • FLJ23841 antibody
    • hVDAC2 antibody
    • OTTHUMP00000019878 antibody
    • OTTHUMP00000019879 antibody
    • OTTHUMP00000019880 antibody
    • Outer mitochondrial membrane protein porin 2 antibody
    • POR antibody
    • Porin-2 antibody
    • VDAC 2 antibody
    • VDAC-2 antibody
    • VDAC2 antibody
    • VDAC2_HUMAN antibody
    • Vdac6 antibody
    • Voltage dependent anion channel 2 antibody
    • Voltage dependent anion selective channel protein 2 antibody
    • Voltage-dependent anion-selective channel protein 2 antibody
    see all

Images

  • Anti-VDAC2 antibody (ab37985) at 1 µg/ml + Human Brain (Cerebellum) Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
  • ICC/IF image of ab37985 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37985, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-VDAC2 antibody (ab37985) at 1 µg/ml + Human Testis lysate (35µg protein in RIPA buffer).

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Tsverava L  et al. Myoinositol Attenuates the Cell Loss and Biochemical Changes Induced by Kainic Acid Status Epilepticus. Biomed Res Int 2016:2794096 (2016). WB . Read more (PubMed: 27642592) »
  • Yuan J  et al. MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals. Autophagy 11:1081-98 (2015). Read more (PubMed: 26060891) »

See all 10 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Unfortunately, both VDAC1 and VDAC3 are very homologous with VDAC2. While we have not tested these antibodies specifically for cross-reactivity with VDAC1 and VDAC3, the antibodies ab37985 and ab118872 show ˜67% homology wi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2017

Application
Western blot
Sample
Rat Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
Mitochondrial fraction from skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - other (Mitochondrial fraction from skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
15 µg
Specification
Mitochondrial fraction from skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 02 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Skeletal muscle)
Loading amount
30 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted Sep 28 2012

Thank you so much.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

We have the following antibodies for VDAC2 and VDAC3 that have been tested in WB and with human samples:

VDAC2: ab47104, ab37985, ab118872, ab100956, ab77160, ab117587, ab126120

VDAC3: ab3745...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
5 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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