MitoSciences (MSA08)

Anti-VDAC/Porin [16G9E6BC4] antibody - Mitochondrial Loading Control (ab110326)

Overview

  • Product nameAnti-VDAC/Porin [16G9E6BC4] antibody - Mitochondrial Loading ControlSee all VDAC/Porin primary antibodies ...
  • Description
    Mouse monoclonal [16G9E6BC4] to VDAC/Porin - Mitochondrial Loading Control
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    VDAC/Porin from S. cerevisiae, denatured in SDS-PAGE.

Properties

Applications

Our Abpromise guarantee covers the use of ab110326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificityHeart, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
  • DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localizationMitochondrion outer membrane. Cell membrane.
  • Target information above from: UniProt accession P21796 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
      • N2441 antibody
      • OMP2 antibody
      • POR1 antibody
      • hVDAC1 antibody
      • MGC111064 antibody
      • Mitochondrial Porin antibody
      • Outer mitochondrial membrane protein porin 1 antibody
      • Plasmalemmal porin antibody
      • Porin 31HL antibody
      • Porin 31HM antibody
      • VDAC antibody
      • VDAC-1 antibody
      • Vdac1 antibody
      • VDAC1_HUMAN antibody
      • Voltage dependent anion channel 1 antibody
      • Voltage dependent anion selective channel protein 1 antibody
      • Voltage-dependent anion-selective channel protein 1 antibody
      • YNL055C antibody
      • YNL2441C antibody
      see all

    Anti-VDAC/Porin [16G9E6BC4] antibody - Mitochondrial Loading Control images

    • Anti-VDAC/Porin [16G9E6BC4] antibody - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrane fraction.

      Predicted band size : 30 kDa

    References for Anti-VDAC/Porin [16G9E6BC4] antibody - Mitochondrial Loading Control (ab110326)

    This product has been referenced in:
    • Chimi MA  et al. Age-related changes in the mitochondrial proteome of the fungus Podospora anserina analyzed by 2D-DIGE and LC-MS/MS. J Proteomics 91:358-74 (2013). Fungi . Read more (PubMed: 23872087) »
    • Trevisson E  et al. Functional complementation in yeast allows molecular characterization of missense argininosuccinate lyase mutations. J Biol Chem 284:28926-34 (2009). Read more (PubMed: 19703900) »

    See all 13 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Prof. Chichung Lie

    Verified customer

    Submitted Aug 01 2013

    Thank you for your enquiry.
    Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"