![Western blot - VDAC/Porin antibody [16G9E6BC4] (ab110326) image](#VDACPorin-Primary-antibodies-ab110326-1.jpg)
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Product name
Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control
See all VDAC/Porin products (9) ...
Description
Mouse monoclonal [16G9E6BC4] to VDAC/Porin - Mitochondrial Loading Control
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
VDAC/Porin from S. cerevisiae, denatured in SDS-PAGE.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab110326 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
16G9E6BC4
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - VDAC/Porin antibody [16G9E6BC4] (ab110326)
![Western blot - VDAC/Porin antibody [16G9E6BC4] (ab110326) image](/ps/datasheet/images/110/ab110326/VDACPorin-Primary-antibodies-ab110326-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
Target
Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificity
Heart, liver and skeletal muscle.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localization
Mitochondrion outer membrane. Cell membrane.
Target information above from: UniProt accessionP21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hVDAC1 antibody
- MSA08 antibody
- Outer mitochondrial membrane protein porin 1 antibody
see all
Database links
- SwissProt: P04840 Saccharomyces cerevisiae
Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control images:
Western blot - VDAC/Porin antibody [16G9E6BC4] (ab110326)
Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326) at 1 µg/ml + Yeast spheroplast membrnae fraction.
Predicted band size : 30 kDa
References for Anti-VDAC/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
This product has been referenced in:
- Trevisson E et al. Functional complementation in yeast allows molecular characterization of missense argininosuccinate lyase mutations. J Biol Chem 284:28926-34 (2009).Read more (PubMed: 19703900) »
- Ukibe K et al. Metabolic engineering of Saccharomyces cerevisiae for astaxanthin production and oxidative stress tolerance. Appl Environ Microbiol 75:7205-11 (2009).Read more (PubMed: 19801484) »
See all 12 publications for this product
Publishing research using ab110326? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"