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Synthetic peptide: NDGTEFGGSIYQK, corresponding to amino acids 185-197 of Human VDAC/Porin.
Our Abpromise guarantee covers the use of ab34726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/50000.|
|WB||1/500 - 1/3000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).|
|ICC||1/100. Fixation with PFA requires a denaturing step, see Abreview by M.Schrader.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"