Overview

  • Product nameAnti-VDAC/Porin antibodySee all VDAC/Porin primary antibodies ...
  • Description
    Mouse monoclonal to VDAC/Porin
  • Tested applicationsWB, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus Monkey, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment with tag: MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE TTKVTGSLET KYRWTEYGLT FTEKWNTDNT LGTEITVEDQ LARGLKLTFD , corresponding to amino acids 1-100 of Human VDAC1/ Porin

Properties

Applications

Our Abpromise guarantee covers the use of ab61273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells.
ELISA Use at an assay dependent dilution.

Target

  • FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificityHeart, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
  • DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localizationMitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31 HL antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC 1 antibody
    • VDAC antibody
    • VDAC-1 antibody
    • VDAC1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Anti-VDAC/Porin antibody images



  • Predicted band size : 31 kDa
  • Overlay histogram showing HepG2 cells stained with ab61273 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61273, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-VDAC/Porin antibody (ab61273)

This product has been referenced in:
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »

See 1 Publication for this product

Product Wall

Thank you for the information regarding the efficacy of the secondary antibody.
I will be happy to send ab14734 as a free-of-charge replacement for ab61273 now, if you like. You will still need to pay for ab61273, if you have not done so already. ...

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Thank you for troubleshooting our complaint form. Your protocol looks very good, and you should be able to detect VDAC with these conditions.

My one concern was the affinity-purified secondary from Jackson Lab, that it was isotype subclass-s...

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Thank you for following up with me, and I'm sorry to hear that the second vial also did not work well.

I am happy to send you a vial of ab14734, and this should arrive early next week on the order ***.

Please keep me updated about t...

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Thank you for your call today, and I am sorry for the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab15895 on the order ***. This antibody is in stock and should arrive tomorrow.

Please keep me u...

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Thank you for your enquiry.
Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer...

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Thank you for your reply. I am sending a free of charge vial of ab61273 from a new lot on the order ***. This product is in stockand it should arrive within a couple of days. Please keep me updated of the results using this replacement, and let me know...

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Thank you for contacting us and letting us know about the trouble with this vial of ab61273. I have looked through our records, and we haven't received any other reports of a similar problem with the lot that you currently have (I found your order and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"