Overview

  • Product name
    Anti-VE Cadherin antibody [3D5C7]
    See all VE Cadherin primary antibodies
  • Description
    Mouse monoclonal [3D5C7] to VE Cadherin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 29-223 of Human VE Cadherin, expressed in E.coli.

  • Positive control
    • Human recombinant VE Cadherin protein; MCF7, A549 and HUVE-12 cell lysates; rat lung lysate; Jurkat cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR268881-11 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab166715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 88 kDa.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificity
    Endothelial tissues and brain.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localization
    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Images

  • Anti-VE Cadherin antibody [3D5C7] (ab166715) at 1/500 dilution + Recombinant Human VE Cadherin protein - The recombinant protein is a fragment: AA: 29-223 containing a His-tag.

    Predicted band size: 88 kDa



    Expected MWt is 47.6 kDa
  • All lanes : Anti-VE Cadherin antibody [3D5C7] (ab166715) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HUVE-12 cell lysate
    Lane 4 : Rat lung tissue lysate

    Predicted band size: 88 kDa

  • Flow cytometry analysis of Jurkat cells labeling ab166715 at 1/200 dilution (green) compared to a negative control (purple).

References

This product has been referenced in:
  • Zhou J  et al. Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration. Stem Cells Dev 26:91-101 (2017). Read more (PubMed: 27832737) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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