Anti-VE Cadherin (phospho Y685) antibody (ab119785)
- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-VE Cadherin (phospho Y685) antibody
See all VE Cadherin primary antibodies - DescriptionRabbit polyclonal to VE Cadherin (phospho Y685)
- Specificityab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Immunogen
Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.
- Positive control
- Human umbilical vein endothelial cells stimulated with pervanadate.
- General notesDo not aliquot.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab119785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa). |
Target
- FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
- Tissue specificityEndothelial tissues and brain.
- Sequence similaritiesContains 5 cadherin domains.
- Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. - Cellular localizationCell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
- Information by UniProt
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Database links
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Entrez Gene: 307618 Rat
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: P55284 Mouse
- Unigene: 76206 Human
- Unigene: 21767 Mouse
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Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Anti-VE Cadherin (phospho Y685) antibody images
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Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785)All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution
Lane 1 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min.
Lane 2 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min then treated with alkaline phosphatase
Predicted band size : 88 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
References for Anti-VE Cadherin (phospho Y685) antibody (ab119785)
This product has been referenced in:
- Lee CS et al. Dipeptidyl Peptidase-4 Inhibitor Increases Vascular Leakage in Retina through VE-cadherin Phosphorylation. Sci Rep 6:29393 (2016). Read more (PubMed: 27381080) »
- Zhang P et al. Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem 291:2087-106 (2016). Read more (PubMed: 26504080) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"