Anti-VE Cadherin (phospho Y685) antibody (ab119785)

Overview

  • Product nameAnti-VE Cadherin (phospho Y685) antibody
    See all VE Cadherin primary antibodies
  • Description
    Rabbit polyclonal to VE Cadherin (phospho Y685)
  • Specificityab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.

  • Positive control
    • Human umbilical vein endothelial cells stimulated with pervanadate.
  • General notesDo not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificityEndothelial tissues and brain.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localizationCell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Anti-VE Cadherin (phospho Y685) antibody images

  • All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

    Lane 1 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min.
    Lane 2 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min then treated with alkaline phosphatase


    Predicted band size : 88 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

References for Anti-VE Cadherin (phospho Y685) antibody (ab119785)

ab119785 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (lung)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 30 µg
Specification lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Sep 09 2016

Thank you for your inquiry.

I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

I would like to assure you that both antibodies are suitable for your purposes.

They are antibodies g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"