Anti-VE Cadherin (phospho Y685) antibody (ab119785)

Abreviews (2)Q&A (1)Specific References (4)

Overview

  • Product name
    Anti-VE Cadherin (phospho Y685) antibody
    See all VE Cadherin primary antibodies
  • Description
    Rabbit polyclonal to VE Cadherin (phospho Y685)
  • Host species
    Rabbit
  • Specificity
    ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.

  • Positive control
    • Human umbilical vein endothelial cells stimulated with pervanadate.
  • General notes
    Do not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificity
    Endothelial tissues and brain.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localization
    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
    • Alternative names
      • 7B 4 antibody
      • 7B4 antibody
      • 7B4 antigen antibody
      • CADH5_HUMAN antibody
      • Cadherin 5 antibody
      • Cadherin 5 type 2 antibody
      • Cadherin 5, type 2 (vascular endothelium) antibody
      • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
      • cadherin, vascular endothelial antibody
      • cadherin, vascular endothelial, 1 antibody
      • Cadherin-5 antibody
      • Cadherin5 antibody
      • CD 144 antibody
      • CD144 antibody
      • CD144 antigen antibody
      • CDH 5 antibody
      • CDH5 antibody
      • CDH5 protein antibody
      • Endothelial specific cadherin antibody
      • FLJ17376 antibody
      • OTTHUMP00000174777 antibody
      • Vascular endothelial cadherin antibody
      • Vascular epithelium cadherin antibody
      • VE Cad antibody
      • VE-cadherin antibody
      • VEC antibody
      see all

    Images

    • Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785)
      Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785)
      All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

      Lane 1 : Human umbilical vein endothelial
      cells stimulated with pervanadate (1 mM) for 30 min.
      Lane 2 : Human umbilical vein endothelial
      cells stimulated with pervanadate (1 mM) for 30 min then treated with
      alkaline phosphatase

      Predicted band size: 88 kDa
      Observed band size: 140 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Yan N  et al. Promotion of anoxia-reoxygenation-induced inflammation and permeability enhancement by nicotinamide phosphoribosyltransferase-activated MAPK signaling in human umbilical vein endothelial cells. Exp Ther Med 14:4595-4601 (2017). Read more (PubMed: 29104667) »
    • Lee CS  et al. Dipeptidyl Peptidase-4 Inhibitor Increases Vascular Leakage in Retina through VE-cadherin Phosphorylation. Sci Rep 6:29393 (2016). Read more (PubMed: 27381080) »

    See all 4 Publications for this product

    Publishing research using ab119785? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-VE Cadherin (phospho Y685) antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (placenta)
    Gel Running Conditions
    Reduced Denaturing (gel: 6% (upper half), 10% (below))
    Loading amount
    30 µg
    Specification
    placenta
    Blocking step
    5% milk + 2% BSA in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 06 2018

    Western blot abreview for Anti-VE Cadherin (phospho Y685) antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    30 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2016

    Dear Sir Or Madam,
    I would like to order an antibody,Anti-VE Cadherin (phospho Y685)
    antibody for western blot with HUVCECs. I found there are two product:
    ab119785 and ab27775. Could you please tell me the difference of these
    two p...

    Read More

    Thank you for your inquiry.

    I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

    I would like to assure you that both antibodies are suitable for your purposes.

    They are antibodies g...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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