Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
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ab14994 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lanes 1 - 2 : clone MRVL56/1 at 1 µg/ml
Lanes 3 - 4 : pan VEGF antibody
Lane 1 : VEGF165B recombinant
Lane 2 : VEGF165 recombinant
Lane 3 : VEGF165B recombinant
Lane 4 : VEGF165 recombinant
Secondary
anti-mouse IgG
developed using the ECL technique
Predicted band size : 22 kDa
Top panel: clone MRVL56/1 used on human renal glomerus at 2ug/ml.
Bottom panel: control IgG primary used on human renal glomerus at 2ug/ml.
ELISA on recombinant VEGF 165B.
Blue - clone MRVL56/1
Red - Pan VEGF antibody
Anti-VEGF 165B antibody [MRVL56/1] (ab14994) at 5 µg/ml + VEGF 165b HNCO2 at 10 µl
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Exposure time : 16 minutes
ICC/IF image of ab14994 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab14994 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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