Overview

  • Product nameVEGF Human ELISA Kit
    See all VEGF kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.5 92% - 113%
    Serum 104.4 92% - 115%
    Plasma 105.7 93% - 114%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceVEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
  • Alternative names
    • MGC70609
    • MVCD1
    • Vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF A
    • VEGFA
    • VPF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

VEGF Human ELISA Kit images

  • VEGF measured in biological fluids showing quantity (pg) per milliliter of tested sample

  • Curves showing concentrations huVEGF165 in NIBSC 02/286 using ab100662 VEGF human

     

  • Representative standard curve using ab100662

  • Representative standard curve using ab100662

Protocols

References for VEGF Human ELISA Kit (ab100662)

This product has been referenced in:
  • Tejero R  et al. miR-141 and miR-200c as markers of overall survival in early stage non-small cell lung cancer adenocarcinoma. PLoS One 9:e101899 (2014). Human . Read more (PubMed: 25003366) »

See 1 Publication for this product

Product Wall



Please find below the information regarding the source/host of our capture and detection antibodies used in our VEGF Human ELISA Kit (ab100662):

Capture Antibody: Monoclonal Mouse IgG2B

Detection Antibody: Polyclonal Goat I...

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Please note that ab100662 and ab100663 are similar kits that both measure levels of VEGF.

However ab100662 is best used for detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma while ab100663 is best sui...

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Unfortunately we no longer test urine as part of the validation. However the kit is very likely to work for urine (based on our past experience with this sample type).

Here are so some general guidelines on preparation of urine for ELISA tes...

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Capture Antibody: Monoclonal, mouse. The immunogen sequence is Ala27­Arg191 (Accession # NP_001165097.1) Detects human VEGF165 and human VEGF121. In ELISAs, this antibody has ˜10% cross­reactivity with recombinant mouse VEGF and rat V...

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The VEGFA isoform that ab100662 detects is 165 and 121.



Answer 1:

Please use 1X Assay Diluent B to dilute the detection antibody and the HRP-Streptavidin. If testing serum/plasma, use Assay Diluent A to prepare/dilute the standards and samples. Where it says use the “appropriate as...

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Thank you for contacting us.

I can confirm that Heparin should not be interfering with the assay using these kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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Te pido disculpas por la espera.

He recibido una contestación de mis compañeros, que me comentan que la dilución recomendada para suero y plasma debe hacerse entre 2 y 5 veces.

En cualquier caso, los niveles de ...

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Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"