VEGF Mouse ELISA Kit (ab100752)
- Product nameVEGF Mouse ELISA KitSee all VEGF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range4.1 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture extracts 88.62 82% - 103% Tissue Extracts 87.87 81% - 102%
- Species reactivityReacts with: Mouse
- Product overview
VEGF (Vascular endothelial growth factor) is also called VEGF-A, following the identification of several VEGF-related factors (VEGF-B, VEGF-C, VEGF-D, VEGF-E). VEGF significantly influences vascular permeability and is a strong angiogenic protein in several Bioassays and probably also plays a role in neovascularisation under physiological conditions. VEGF plays a role in the development and function of primate follicles and the ovarian corpus luteum, supporting the proliferation of blood vessels. The differentiation of adipocytes, of pheochromocytomas, and myocytes is accompanied by the controlled expression of VEGF. It has been demonstrated that inhibition of VEGF activity by treatment with a monoclonal antibody specific for VEGF can suppress tumor growth in vivo.
ab100752 Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF cell lysate and tissue lysate. This assay employs an antibody specific for mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml Detection Antibody VEGF: biotinylated anti-mouse VEGF 2 vials HRP-Streptavidin Concentrate 160X Item G 1 x 200µl Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml VEGF Microplate (12 strips x 8 wells) coated with anti-mouse VEGF 1 unit VEGF Standard Mouse (recombinant) 2 vials
- Research Areas
- MGC70609MVCD1Vascular endothelial growth factor
- Vascular endothelial growth factor AVascular permeability factorVEGF AVEGFAVPF
- SwissProt: Q00731 Mouse
Our Abpromise guarantee covers the use of ab100752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for VEGF Mouse ELISA Kit (ab100752)
ab100752 has not yet been referenced specifically in any publications.