Overview

  • Product nameAnti-VEGF Receptor 1 antibody
    See all VEGF Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 1
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Electron Microscopy, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide (Human) C-terminal.

  • Positive control
    • Hey cells. Angiosarcoma. ICC/IF: A431 cell line
  • General notes


    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.

Properties

Applications

Our Abpromise guarantee covers the use of ab2350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/25 - 1/50. Fix with acetone. Incubate overnight at 4C.
Electron Microscopy 1/100. PubMed: 18647955
WB Use at an assay dependent concentration. PubMed: 21912702
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • Tissue specificityMostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Anti-VEGF Receptor 1 antibody images

  • ab2350 - immunohistochemistry

    Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.

  • ab2350 staining the VEGF Receptor 1 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab2350 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-VEGF Receptor 1 antibody (ab2350)

This product has been referenced in:
  • Kanthou C  et al. Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics. PLoS One 9:e104015 (2014). WB ; Mouse . Read more (PubMed: 25119572) »
  • Majeti BK  et al. VEGF is an important mediator of tumor angiogenesis in malignant lesions in a genetically engineered mouse model of lung adenocarcinoma. BMC Cancer 13:213 (2013). IHC-P ; Mouse . Read more (PubMed: 23627488) »

See all 18 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 12 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the product ab2350 did not perform as stated on the datasheet and for the inconvenienc...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jun 11 2010

Thank you for your enquiry. This antibody has not been tested on canine tissues. We do not give discounts on products in order to test the antibody, but we do have a rewards program. If you use the antibody on canine tissue, please consider submitting ...

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Thank you for all the information you have provided, I think the following recommendations will help and I am also waiting for last pieces of information from the lab which will help too. Firstly I think it is very important that we establish if VEG...

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I'm very sorry to hear you are experiencing a problem with ab2350. I have consulted other colleagues to see if we could detect a possible protocol problem and can confirm that your protocol seems fine to us. The only thing we are suspecting is that the...

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Thank you for your email. I'm sorry that my protocol suggestions did not help you and I can offer to send you replacement vials from a newer batch free of charge. Can you please tell me the Abcam order number or purchase order that was used when you or...

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with these two antibodies. At this point I would like to make the following suggestions. Please use an amplification step, such as the ABC method, in order to ampli...

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Thank you for your e-mail. Amplification is a method to amplify signal on immunohistochemical preparations where there is low abundance of the protein of interest (or the primary antibody binding affinity for the protein is low). The most common ...

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I'm sorry to hear you are having problems with ab2350. We recommend the following on our datasheet for the immunohistochemistry application on paraffin embedded sections: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We su...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"