VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
Our Abpromise guarantee covers the use of ab2350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Electron Microscopy||1/100. PubMed: 18647955|
|WB||Use at an assay dependent concentration. PubMed: 21912702|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.