Anti-VEGF Receptor 1 antibody [Flt-1/EWC] (ab9540)


  • Product nameAnti-VEGF Receptor 1 antibody [Flt-1/EWC]
    See all VEGF Receptor 1 primary antibodies
  • Description
    Mouse monoclonal [Flt-1/EWC] to VEGF Receptor 1
  • SpecificityDetects native and denaturated VEGFR-1/ Flt-1
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Recombinant human soluble extracellular Flt-1 Ig-like loop 1 to 5 (sFlt-1(D5)).

  • Positive control
    • recombinant soluble VEGFR 1, soluble human VEGFR-1 from human follicular fluids (see Neulen et al), mouse placenta tissue
  • General notes

    VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.



Our Abpromise guarantee covers the use of ab9540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 2 - 5 µg/ml.
ICC/IF 1/50.
IHC-P Use at an assay dependent concentration. PubMed: 17823277
Flow Cyt Use at an assay dependent concentration. PubMed: 25223735


  • FunctionReceptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • Tissue specificityMostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Anti-VEGF Receptor 1 antibody [Flt-1/EWC] images

  • Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of Human Placenta, Placental Bed and Decidua Parietalis. VEGF Receptor 1 (geen) was labelled with ab9540 and VEGF Receptor 2 (red) was labelled with ab2349. Yellow - where both VEGF Receptor 1 and VEGF Receptor 2 are expressed. DAPI was used for nuclei staining (blue).

References for Anti-VEGF Receptor 1 antibody [Flt-1/EWC] (ab9540)

This product has been referenced in:
  • Ostyn P  et al. Transient TNF regulates the self-renewing capacity of stem-like label-retaining cells in sphere and skin equivalent models of melanoma. Cell Commun Signal 12:52 (2014). Flow Cyt ; Human . Read more (PubMed: 25223735) »
  • Suspitsin EN  et al. Evidence for angiogenesis-independent contribution of VEGFR1 (FLT1) in gastric cancer recurrence. Med Oncol 30:644 (2013). Read more (PubMed: 23801279) »

See all 14 Publications for this product

Product Wall

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for your message. I can confirm that the ab9540 VEGF Receptor 1 antibody [Flt-1/EWC] was generated using the recombinant soluble FLT-1 (domain 1-5) for immunization of mouse. Therefore it detects the soluble as well as the transmembran...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (endothelial cells)
Specification endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton/0.1% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 17 2008

I'm sorry to hear you are having a problem with ab9540. I checked the enquiries regarding this antibody and I didn't see any other complaints of differences between lots since November of last year. Since you have used this antibody successfully ...

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Thank you for your enquiry. The binding epitope is located in ecto domain D1 or D2. It is not further characterized. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. We do not know if the antibody recognizes the receptor when bound with its ligand. We can confirm that the antibody recognizes the receptor unbound as the antibody will detect denaturated human VEGFR-1/ Flt-1 in ELISA experi...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab9540. It is strange that you are not seeing any signal at all with the HUVEC sample and you are with the human placenta trophoblast samples. You may need to lo...

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Thank you for submitting for your complaint and for providing more details of your protocol. Looking at our database, we can see that we have sold several vials of batch: 68526 without any problem. It may well be that the vial you received has just...

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Thank you for your enquiry and your patience. We would like to confirm that this antibody recognizes both forms. IP is a normal standard protocol: 1-2µg antibody, 30µl anti-mouse IgG activated Sepharose beads (Sigma), incubation for 3-4 hours o...

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To our knowledge this antibody reacts with mouse 3T3 cells transfected with the mouse flt-1 cDNA in Western blotting. This was shown by Dr. Georg Breier (MPI Bad Nauheim, Germany). We are not sure whether the endogenous expression of Flt-1 or sFlt-1 i...

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