Recombinant
RabMAb

Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab32152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 151 kDa.
IHC-P 1/250.
IP 1/100.
IHC-Fr 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
    • Tissue specificity
      Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.1 antibody
      • FLT 1 antibody
      • FLT antibody
      • Flt-1 antibody
      • FLT1 antibody
      • Fms like tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
      • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
      • Fms-like tyrosine kinase 1 antibody
      • FRT antibody
      • Soluble VEGF receptor 1 14 antibody
      • Soluble VEGFR1 variant 2 antibody
      • Soluble VEGFR1 variant 21 antibody
      • Tyrosine protein kinase FRT antibody
      • Tyrosine protein kinase receptor FLT antibody
      • Tyrosine-protein kinase FRT antibody
      • Tyrosine-protein kinase receptor FLT antibody
      • Vascular endothelial growth factor receptor 1 antibody
      • Vascular endothelial growth factor vascular permeability factor receptor antibody
      • Vascular permeability factor receptor 1 antibody
      • Vascular permeability factor receptor antibody
      • VEGFR 1 antibody
      • VEGFR-1 antibody
      • VEGFR1 antibody
      • VGFR1_HUMAN antibody
      see all

    Anti-VEGF Receptor 1 antibody [Y103] images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse brain tissue section (15 days old wild-type mouse embryonic brain, 16 micron) labeling VEGF Receptor 1 with ab32152 at 1/300 dilution. Tissue was fixed with formaldehyde and permeabilized with Triton X100. Heat mediated antigen retrieval was performed using 10mM citrate buffer, pH 6. A polyclonal donkey anti-Rabbit IgG (H+L) (Alexa Fluor® 488) secondary antibody was used at 1/500 dilution. 

      See Abreview

    • Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue

      Predicted band size : 151 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

      Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

    References for Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    This product has been referenced in:
    • Stone OA  et al. Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling. J Physiol 595:1575-1591 (2017). WB ; Rat . Read more (PubMed: 27868196) »
    • Kim YS  et al. Repeated Microneedle Stimulation Induces Enhanced Hair Growth in a Murine Model. Ann Dermatol 28:586-592 (2016). IHC-P ; Mouse . Read more (PubMed: 27746638) »

    See all 72 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (carnuncle)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    50 µg
    Specification
    carnuncle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mrs. Gail Silver

    Verified customer

    Submitted May 13 2017

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
    Sample
    Mouse Tissue sections (15 days old WT embryonic brain, 16 micron)
    Specification
    15 days old WT embryonic brain, 16 micron
    Permeabilization
    Yes - Triton X100
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 06 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (liver, Hep G2)
    Specification
    liver, Hep G2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 06 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (liver, hepatocytes)
    Specification
    liver, hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (HUVECs)
    Specification
    HUVECs
    Treatment
    10ng/ml IL-1 beta
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Tissue lysate - whole (Placenta)
    Specification
    Placenta
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
    Username

    Vicky

    Verified customer

    Submitted Jan 30 2014

    ab32152 was tested with a permeabilization step but the immunogen sequence and SwissProt suggests an extracellular location. Whilst collating testing conditions, permeabilization was assigned based on “Secreted and Cell membrane” from the c...

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    After looking into our data for ab32152, I found the following abReview, which was submitted by another researcher using ab32152 in immunocytochemistry. According to the researcher's protocol (link below) permeabilization was not necessary, which ...

    Read More

    Thank you for your email.

    The concentration of the ab is 15ug/ml which means 0.015 ug/ul.

    The 100ul of antibody will concentration 1.5ug per 100ul.

    This antibody is rabbit monoclonal antibody which is different in specifici...

    Read More

    Thank you for contacting us.

    The concentration of lot is GR106697 is 15 ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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