Recombinant
RabMAb

Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab32152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 151 kDa.
IHC-P 1/250.
IP 1/100.
IHC-Fr 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
    • Tissue specificity
      Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.1 antibody
      • FLT 1 antibody
      • FLT antibody
      • Flt-1 antibody
      • FLT1 antibody
      • Fms like tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
      • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
      • Fms-like tyrosine kinase 1 antibody
      • FRT antibody
      • Soluble VEGF receptor 1 14 antibody
      • Soluble VEGFR1 variant 2 antibody
      • Soluble VEGFR1 variant 21 antibody
      • Tyrosine protein kinase FRT antibody
      • Tyrosine protein kinase receptor FLT antibody
      • Tyrosine-protein kinase FRT antibody
      • Tyrosine-protein kinase receptor FLT antibody
      • Vascular endothelial growth factor receptor 1 antibody
      • Vascular endothelial growth factor vascular permeability factor receptor antibody
      • Vascular permeability factor receptor 1 antibody
      • Vascular permeability factor receptor antibody
      • VEGFR 1 antibody
      • VEGFR-1 antibody
      • VEGFR1 antibody
      • VGFR1_HUMAN antibody
      see all

    Anti-VEGF Receptor 1 antibody [Y103] images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse brain tissue section (15 days old wild-type mouse embryonic brain, 16 micron) labeling VEGF Receptor 1 with ab32152 at 1/300 dilution. Tissue was fixed with formaldehyde and permeabilized with Triton X100. Heat mediated antigen retrieval was performed using 10mM citrate buffer, pH 6. A polyclonal donkey anti-Rabbit IgG (H+L) (Alexa Fluor® 488) secondary antibody was used at 1/500 dilution. 

      See Abreview

    • Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue

      Predicted band size : 151 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

      Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

    References for Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    This product has been referenced in:
    • Stone OA  et al. Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling. J Physiol 595:1575-1591 (2017). WB ; Rat . Read more (PubMed: 27868196) »
    • Wang Q  et al. Compound anisodine affects the proliferation and calcium overload of hypoxia-induced rat retinal progenitor cells and brain neural stem cells via the p-ERK1/2/HIF-1a/VEGF pathway. Exp Ther Med 14:600-608 (2017). Read more (PubMed: 28672973) »

    See all 76 Publications for this product

    Product Wall

    ab32152 was tested with a permeabilization step but the immunogen sequence and SwissProt suggests an extracellular location. Whilst collating testing conditions, permeabilization was assigned based on “Secreted and Cell membrane” from the c...

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    After looking into our data for ab32152, I found the following abReview, which was submitted by another researcher using ab32152 in immunocytochemistry. According to the researcher's protocol (link below) permeabilization was not necessary, which ...

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    Thank you for your email.

    The concentration of the ab is 15ug/ml which means 0.015 ug/ul.

    The 100ul of antibody will concentration 1.5ug per 100ul.

    This antibody is rabbit monoclonal antibody which is different in specifici...

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    Thank you for contacting us.

    The concentration of lot is GR106697 is 15 ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Vielen Dank für Ihre Anfrage.

    Bei dem Anti-VEGF Receptor 1 antibody [Y103] (ab32152) handelt es sich um einen Antikoerper in "Tissue culture supernatant". Dieser wurde aufgereinigt, so dass nur noch Proteine mit der IgG Fraktion enthalten sind...

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    Thank you for your inquiry.

    I can confirm that the concentration of the lot (GR29967-2)that is in stock right now is 0.023mg/ml.

    I also would like to comment on the seemingly low concentration:

    The IgG produced by rabbits ...

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    Thank you for completing our follow up survey.

    I am sorry that the protocol tips I provided did not prove to be successful. Under our Abrpromise we guarantee this antibody to work in IHC on mouse tissue and since it is not working for you I w...

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    Thank you for contacting Abcam.

    I am sorry that you are having problems with ab32152 in IHC-P on mouse skeletal muscle tissue. I have looked over the protocol that you sent and I would recommend that you use an antigen retrieval step with thi...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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    Vielen Dank für Ihre Anfrage. Die Konzentration fuer lot GR29967-1 ist 23 ug/ml (0.023 mg/ml). The concentration is 23 ug/ml (0.023 mg/ml). Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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