Recombinant
RabMAb

Anti-VEGF Receptor 1 antibody [Y103] (HRP) (ab194714)

Overview

  • Product name
    Anti-VEGF Receptor 1 antibody [Y103] (HRP)
    See all VEGF Receptor 1 primary antibodies
  • Description
    Rabbit monoclonal [Y103] to VEGF Receptor 1 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in N terminus of human VEGF Receptor 1

  • Positive control
    • WB: Mouse Brain.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab194714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 151 kDa).

Target

  • Function
    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • Tissue specificity
    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Images

  • Anti-VEGF Receptor 1 antibody [Y103] (HRP) (ab194714) at 1/5000 dilution + Brain (Mouse) Tissue Lysate at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 151 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194714 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab194714 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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