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KMVDAAVHADSGTTLQLTSC, corresponding to C terminal amino acids 1326-1345 of Mouse VEGF Receptor 2.
Isoform 1 localization: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.
Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.
Our Abpromise guarantee covers the use of ab2349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18602918|
ab2349 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 2, using ABC and AEC chromogen.
ab2349 at a 1/100 dilution staining Human choroidal endothelial cells by Immunocytochemistry. The antibody was incubated with the cells for 3 hours and then detected using an Alexa-Fluor 488® goat anti-rabbit (IgG).
This image is courtesy of an Abreview submitted on 27 February 2006.
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