• Product nameAnti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2
  • Tested applicationsSuitable for: IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 1326-1345 of Mouse VEGF Receptor 2.

  • Positive control
    • Angiosarcoma.
  • General notes

    Isoform 1 localization: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.

    Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.


Our Abpromise guarantee covers the use of ab2349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration. See Abreview.
Flow Cyt Use at an assay dependent concentration. PubMed: 18602918

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 antibody images

  • ab2349 - immunohistochemistry

    Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 2, using ABC and AEC chromogen.

  • ab2349 at a 1/100 dilution staining Human choroidal endothelial cells by Immunocytochemistry. The antibody was incubated with the cells for 3 hours and then detected using an Alexa-Fluor 488® goat anti-rabbit (IgG).

    This image is courtesy of an Abreview submitted on 27 February 2006.

    See Abreview

  • ab2349 staining the VEGF Receptor 2 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-VEGF Receptor 2 antibody (ab2349)

This product has been referenced in:
  • Li W  et al. Preliminary in vitro and in vivo assessment of a new targeted inhibitor for choroidal neovascularization in age-related macular degeneration. Drug Des Devel Ther 10:3415-3423 (2016). WB ; Human . Read more (PubMed: 27799741) »
  • El-Gendy R  et al. Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass® Scaffolds. Tissue Eng Part A 21:2034-43 (2015). IHC-P . Read more (PubMed: 25923923) »

See all 45 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Granulation tissue)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Granulation tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 25°C
Fixative Formaldehyde

Francisco Fernandez Flores

Verified customer

Submitted Dec 01 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (iCell® Cardiomyocytes)
Permeabilization No
Specification iCell® Cardiomyocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Acetone

Abcam user community

Verified customer

Submitted Nov 25 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Rat Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 10 2014

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The Anti-VEGF Receptor 2 antibody (ab2349) has only so far been validated inhouse with human tissue in IHC. ...

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