Overview

  • Product nameAnti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human VEGF Receptor 2.

    (Peptide available as ab39255.)

  • Positive control
    • This product gave a positive signal in HUVEC whole cell lysate.

Applications

Our Abpromise guarantee covers the use of ab39256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 152 kDa).Can be blocked with Human VEGF Receptor 2 peptide (ab39255).

Target

  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 antibody images

  • Anti-VEGF Receptor 2 antibody (ab39256) at 1 µg/ml + HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 152 kDa
    Observed band size : 220,230 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab39256 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab39256 staining VEGF Receptor 2 in Human breast carcinoma tissue sections by IHC-P (Formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5 minutes of peroxidase block followed by 10 minutes of protein block at 20°C; antigen retrieval was heat mediated in retrieval solution. Samples were incubated with primary antibody (1/250 in antibody diluent) for 45 minutes at 20°C. An undiluted HRP-conjugated polymer goat anti-mouse/rabbit IgG polyclonal polymer was used as secondary antibody.

    See Abreview

  • ab39256 staining VEGF Receptor 2 in rat heart tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation with 4% PFA and it was later postfixed overnight in the same fixative. It was then cryoprotected in 30% sucrose and cut using a cryostat. The primary antibody was diluted 1/100 and incubated with sample for 18 hours at 20°C in PBS + 0.3 % Triton X100. An Alexa Fluor® 488 - conjugated goat polyclonal to rabbit IgG was used at dilution at 1/1000 as secondary antibody.

    See Abreview

References for Anti-VEGF Receptor 2 antibody (ab39256)

This product has been referenced in:
  • Choi SH  et al. Dual-function synthetic peptide derived from BMP4 for highly efficient tumor targeting and antiangiogenesis. Int J Nanomedicine 11:4643-4656 (2016). IHC-P ; Human . Read more (PubMed: 27695323) »
  • Shi J & Wei PK Interleukin-8: A potent promoter of angiogenesis in gastric cancer. Oncol Lett 11:1043-1050 (2016). ICC/IF ; Human . Read more (PubMed: 26893688) »

See all 14 Publications for this product

Product Wall

Application Western blot
Sample Sheep Tissue lysate - whole (Lung)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 20 µg
Specification Lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Miss. Maryline Gomes

Verified customer

Submitted Jul 08 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (5% gel)
Sample Cow Tissue lysate - other (Corpus luteum)
Specification Corpus luteum
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

サノ

Verified customer

Submitted Jan 23 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela, Hep G2)
Specification Hela, Hep G2
Treatment Na3VO4 (sodium orthovanadate) 0.3nM 1 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 02 2015

According to our records, ab39256, ab63319, ab77258, ab86673, ab103674 was proving difficult to use in [APPL] and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more detail...

Read More

Thank you for your email.

We will look forward to receive the images. On the optimization note we strongly recommend to include the blocking step in protocol; please try 5%BSA or 5-10% normal serum for 1-2 hours at room temperature with specie...

Read More

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

As requested, I have issued a free of charge replacement with the order number 1076421 for the VE...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Mar 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jul 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293 U251 Ms Brain PC12)
Loading amount 30 µg
Specification HEK293 U251 Ms Brain PC12
Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Sep 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"