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Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human VEGF Receptor 2.
(Peptide available as ab39255.)
Our Abpromise guarantee covers the use of ab39256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 152 kDa).Can be blocked with Human VEGF Receptor 2 peptide (ab39255).|
ab39256 staining VEGF Receptor 2 in rat heart tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation with 4% PFA and it was later postfixed overnight in the same fixative. It was then cryoprotected in 30% sucrose and cut using a cryostat. The primary antibody was diluted 1/100 and incubated with sample for 18 hours at 20°C in PBS + 0.3 % Triton X100. An Alexa Fluor® 488 - conjugated goat polyclonal to rabbit IgG was used at dilution at 1/1000 as secondary antibody.