Synthetic phospho peptide (Human)containing Tyrosines 1054 and 1059. This sequence is conserved in mouse and rat.
Our Abpromise guarantee covers the use of ab5473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 20566746|
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 200 kDa.|
ab5473 staining VEGF Receptor 2 (phospho Y1054 + Y1059) in 90% confluent log phase Human umbilical vein endothelial cells (panel a) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2 ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue with DAPI (panel b), F-actin stained red with Alexa Fluor® 555 Rhodamine Phalloidin (panel c), merged image shown in panel d.