Anti-VEGF Receptor 2 (phospho Y1059) antibody (ab63472)

Overview

  • Product nameAnti-VEGF Receptor 2 (phospho Y1059) antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2 (phospho Y1059)
  • Specificityab63472 detects endogenous levels of VEGF Receptor 2 only when phosphorylated at tyrosine 1059 (Human: Tyr1059; Mouse: Tyr1057; Rat: Tyr1055).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human VEGF Receptor 2 around the phosphorylation site of tyrosine 1059 (P-D-YP-V-R).

  • Positive control
    • HepG2 cell extracts treated with Na3VO4 (0.3nM, 40mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab63472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 152 kDa).

Target

  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 (phospho Y1059) antibody images

  • All lanes : Anti-VEGF Receptor 2 (phospho Y1059) antibody (ab63472) at 1/500 dilution

    Lane 1 : HepG2 cell extracts treated with Na3VO4 (0.3nM, 40mins)
    Lane 2 : HepG2 cell extracts treated with Na3VO4 (0.3nM, 40mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 152 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)

References for Anti-VEGF Receptor 2 (phospho Y1059) antibody (ab63472)

ab63472 has not yet been referenced specifically in any publications.

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