• Product nameAnti-VEGFA antibody
    See all VEGFA primary antibodies
  • Description
    Rabbit polyclonal to VEGFA
  • Tested applicationsSuitable for: Sandwich ELISA, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Rat VEGFA.



Our Abpromise guarantee covers the use of ab70612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Indirect ELISA
  • Application notesI-ELISA: Use at a concentration of 0.5 - 2.0 µg/ml. Used in conjunction with compatible secondary reagents, ab70612 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

    sELISA: Use at a concentration of 0.5 - 2.0 µg/ml.

    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
    • Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
    • Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
    • Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    • Information by UniProt
    • Database links
    • Alternative names
      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    References for Anti-VEGFA antibody (ab70612)

    ab70612 has not yet been referenced specifically in any publications.

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    We do actually recommend storing the lyophilised antibody at -20C for long term storage; this will be stable for 2 years.

    The antibody should be stored lyophilised at -20C till use and then reconstituted...

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