Overview

  • Product nameAnti-VEGFB antibody
    See all VEGFB primary antibodies
  • Description
    Goat polyclonal to VEGFB
  • SpecificityAnti-human VEGFB exhibits less than 5% cross-reactivity with recombinant human VEGFD and no cross-reactivity with recombinant human VEGF and recombinant mouse VEGF.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length VEGFB 186 (Human), expressed in E. coli.

  • General notesDo not store in a frost-free freezer. Endotoxin level is < 30 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 0.3 ng/well. The detection limit for recombinant human VEGFB 186 is approximately 0.03 ng/well.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 25 ng/lane under non-reducing and reducing conditions. The detection limit for recombinant human VEGFB 186 is approximately 5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 22 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGrowth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
    • Tissue specificityExpressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
    • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
    • Post-translational
      modifications
      VEGF-B186 is O-glycosylated.
    • Cellular localizationSecreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • Vascular endothelial growth factor B antibody
      • VEGF B antibody
      • VEGF related factor antibody
      • VEGF-B antibody
      • VEGF-related factor antibody
      • Vegfb antibody
      • VEGFB_HUMAN antibody
      • VEGFL antibody
      • VRF antibody
      see all

    References for Anti-VEGFB antibody (ab10965)

    ab10965 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"