Overview

  • Product nameAnti-VEGFB antibodySee all VEGFB primary antibodies ...
  • Description
    Rabbit polyclonal to VEGFB
  • Specificityab58461 detects endogenous levels of total VEGFB protein.
  • Tested applicationsWB, ELISA, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human VEGFB.

  • Positive control
    • Extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab58461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/20000.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab76863 as Capture.

Target

  • FunctionGrowth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • Tissue specificityExpressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    VEGF-B186 is O-glycosylated.
  • Cellular localizationSecreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • Target information above from: UniProt accession P49765 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • vascular endothelial growth factor B antibody
    • Vascular endothelial growth factor B precursor antibody
    • VEGF B antibody
    • VEGF related factor antibody
    • VEGF-B antibody
    • VEGF-related factor antibody
    • Vegfb antibody
    • VEGFB_HUMAN antibody
    • VEGFL antibody
    • VRF antibody
    see all

Anti-VEGFB antibody images

  • Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.
  • All lanes : Anti-VEGFB antibody (ab58461) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunizing peptide


    Predicted band size : 22 kDa
    Observed band size : 22 kDa

Protocols

References for Anti-VEGFB antibody (ab58461)

This product has been referenced in:
  • Guan W  et al. Vascular protection by Angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke. PLoS One 6:e24551 (2011). WB ; Rat . Read more (PubMed: 21912702) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (cardiomyocytes)
Loading amount 30 µg
Specification cardiomyocytes
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"