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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human VEGFC.
(Peptide available as ab97415.)
Our Abpromise guarantee covers the use of ab63221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|Flow Cyt||Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
In human spleen, lymph node and thymus tissue lysates, this antibody detects a band close to 47-kDa, corresponding to the predicted MW of VEGF-C. In cell lysates, the antibody detects the same 47-kDa, as well as additional bands around 50-kDa and 37-kDa. The target protein can be glycosylated and the sequence contains both a 31 residue signal sequence and two pro-peptides (residues 32-111 and 228-419). VEGF-C undergoes a complex proteolytic maturation which generates a variety of processed secreted forms.Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab63221 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"