Overview

  • Product nameAnti-Versican antibody
    See all Versican primary antibodies
  • Description
    Rabbit polyclonal to Versican
  • SpecificityThis antibody can be used to detect versican V1 (ADAMTS-1/4 site). This product is against the neoepitope of versican generated by cleavage of the versican core protein by ATAMTS 1/4.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: CGG- DPEAAE, corresponding to amino acids 436-441 of Human Versican (Peptide available as ab39784.)

Properties

Applications

Our Abpromise guarantee covers the use of ab19345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with Human Versican peptide (ab39784).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18641286

Target

  • FunctionMay play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
  • Tissue specificityCerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
  • Involvement in diseaseDefects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
  • Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Developmental stageDisappears after the cartilage development.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 2 antibody
    • Chondroitin sulfate proteoglycan core protein 2 antibody
    • Chondroitin sulfate proteoglycan core protein, cartilage antibody
    • CSPG2 antibody
    • CSPG2_HUMAN antibody
    • ERVR antibody
    • GHAP antibody
    • Glial hyaluronate binding protein antibody
    • Glial hyaluronate-binding protein antibody
    • Large fibroblast proteoglycan antibody
    • PG-M antibody
    • PGM antibody
    • VCAN antibody
    • Versican antibody
    • Versican core protein antibody
    • Versican proteoglycan antibody
    • WGN 1 antibody
    • WGN antibody
    • WGN1 antibody
    see all

Anti-Versican antibody images

  • Anti-Versican antibody (ab19345) at 1 µg/ml + Lysates prepared from human aorta.ab19345 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing Versican from mouse cumulus oocyte complexes.

References for Anti-Versican antibody (ab19345)

This product has been referenced in:
  • Kumar S  et al. Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE(-/-) mice. Sci Rep 6:31130 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27491335) »
  • Demircan K  et al. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. Biomed Res Int 2014:693746 (2014). WB ; Mouse . Read more (PubMed: 25101296) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - other (Aorta)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 5 µg
Treatment PNGaseF
Specification Aorta
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Javier Barallobre-Barreiro

Verified customer

Submitted Jun 24 2015

Thank you for contacting Abcam regarding the anti-Versican antibody ab19345.

Upon further investigation on how to use this antibody in IHC, I would like to recommend the following publications for your reference. We do not routinely test the ...

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Thank you for contacting Abcam regarding ab13945.


I have contacted the originator of this antibody and unfortunately I do not have any additional information regarding its use as a total measure of versican in the cell, as it will only re...

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Vielen Dank für Ihren Anruf.



Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hoh...

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Thank you for contacting us.

Please find attached the WB protocol;

http://www.abcam.com/index.html?pageconfig=resource&rid=13045

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. T...

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Thank you for contacting us. The immunogen for the antibody was CGG-DPEAAE. The CGG residues are part of the immunogen but not of the protein itself and the immunogen sequence matches to the isoform V1 of human versican protein,residues436-441. The ant...

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Thank you for your enquiry. Have you checked the references linked to the datasheet for ab19345? I've attached a PDF file of one of these references where ab19345 was used for IHC-P. I hope this helps. Please let me know if you continue to have trouble...

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Thanks for your reply. I am sorry to hear you have received less than the expected volume in the vial of ab19345 you received. If you have received the product within the last 6 months or so I would be happy to send you a replacement vial. If so, pleas...

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Thank you for the additional information. I see that you have purchased ab19345, Anti-Versican antibody. You should have received 50ul volume as the antibody is at 2ug/ul. I suggest centrifuging the vial to bring all product to the bottom prior to usin...

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Thank you for your enquiry and interest in our products. I can confirm that TIMP2 protein was isolated from mouse lung fibroblasts so the amino acid sequence corresponds to the murineTIMP2. According to the datasheet ab39315 has not been tested for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"