1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).
FunctionGeneral vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Sequence similaritiesBelongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.
DomainComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational modificationsPhosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
ab102470 at 1/500 dilution staining Vesicle docking protein p115 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Vesicle docking protein p115 antibody (ab102470)The image is courtesy of an anonymous Abreview.
ab102470 staining Vesicle docking protein p115 in human HeLa cells using ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22oC. Samples were incubated with primary antibody (1/50 in 1% donkey serum in 0.1% PBST) for 3 hours at 22oC. Alexa Fluor® 594 conjugated Donkey-anti-Rb polyclonal (1/200) was used as the secondary antibody.
References for Anti-Vesicle docking protein p115 antibody (ab102470)
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