Overview

  • Product name
    Anti-Vesicle docking protein p115 antibody
    See all Vesicle docking protein p115 primary antibodies
  • Description
    Rabbit polyclonal to Vesicle docking protein p115
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Vesicle docking protein p115 aa 717-962. The exact sequence is proprietary.
    Database link: O60763

  • Positive control
    • 293T , A431 , HeLa , HepG2 whole cell lysates, A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab184014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities
    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain
    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization
    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle-docking protein antibody
    see all

Images

  • All lanes : Anti-Vesicle docking protein p115 antibody (ab184014) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 108 kDa



    7.5% SDS PAGE

  • Immunocytochemical analysis of methanol fixed A431 cells labeling Vesicle docking protein p115 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.

References

This product has been referenced in:
  • Zhong B  et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev 2015:606934 (2015). WB . Read more (PubMed: 26199678) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184014.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up