Anti-Vesicle docking protein p115 antibody (ab40822)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).

Target

  • Function
    General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • Sequence similarities
    Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • Domain
    Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • Cellular localization
    Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle-docking protein antibody
    see all

Images

  • Anti-Vesicle docking protein p115 antibody (ab40822) at 1 µg/ml + Human kidney lysate - 35µg protein in RIPA buffer

    Secondary
    anti-goat-HRP at 1/3000 dilution

    Predicted band size : 108 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References

ab40822 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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