The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
FunctionGeneral vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Sequence similaritiesBelongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.
DomainComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational modificationsPhosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.