Anti-Vesicle docking protein p115 antibody [EPR2140Y] (ab68137)

Overview

  • Product nameAnti-Vesicle docking protein p115 antibody [EPR2140Y]
    See all Vesicle docking protein p115 primary antibodies
  • Description
    Rabbit monoclonal [EPR2140Y] to Vesicle docking protein p115
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Vesicle docking protein p115

  • Positive control
    • HeLa cell lysate; 293T cell lysate; HepG2 cell lysate; Cos cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab68137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
IP 1/30.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionGeneral vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
    • Sequence similaritiesBelongs to the VDP/USO1/EDE1 family.
      Contains 10 ARM repeats.
    • DomainComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
    • Post-translational
      modifications
      Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
    • Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • General vesicular transport factor antibody
      • General vesicular transport factor p115 antibody
      • P115 antibody
      • Protein USO1 homolog antibody
      • TAP antibody
      • Transcytosis associated protein antibody
      • Transcytosis-associated protein antibody
      • Uso1 antibody
      • USO1_HUMAN antibody
      • VDP antibody
      • Vesicle docking protein antibody
      • Vesicle-docking protein antibody
      see all

    Anti-Vesicle docking protein p115 antibody [EPR2140Y] images

    • All lanes : Anti-Vesicle docking protein p115 antibody [EPR2140Y] (ab68137) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Cos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 108 kDa
      Observed band size : 108 kDa

    References for Anti-Vesicle docking protein p115 antibody [EPR2140Y] (ab68137)

    This product has been referenced in:
    • Jin Y & Dai Z USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells. Biomed Pharmacother 78:264-71 (2016). WB ; Human . Read more (PubMed: 26898451) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"