Vesicular Acetylcholine Transporter antibody (ab31544)

Product Name 

Vesicular Acetylcholine Transporter antibody

Product type 

Primary antibodies

Description 

Sheep polyclonal to Vesicular Acetylcholine Transporter

Immunogen 

Synthetic peptide: C-TRSRSERDVLLDEPPQGLYDAVRLRE, corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.
BLAST 'C-TRSRSERDVLLDEPPQGLYDAVRLRE' with or

Reacts with 

Rat, Gpig

Predicted to react 
(species key)

Hu, Ms, Chmp, Dog, Mcq
(Due to sequence homology)

Specificity 

Ab31544 is specific to vesicular acetylcholine transporter.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Plasma membrane

Research areas 

Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology
Neuroscience >> Neurotransmitter >> Acetylcholine

Relevance 

Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate.
VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.

Database links 

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

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