Anti-Vesicular Acetylcholine Transporter antibody (ab31544)

Overview

  • Product nameAnti-Vesicular Acetylcholine Transporter antibody
    See all Vesicular Acetylcholine Transporter primary antibodies
  • Description
    Sheep polyclonal to Vesicular Acetylcholine Transporter
  • SpecificityAb31544 is specific to vesicular acetylcholine transporter.
  • Tested applicationsSuitable for: IHC - Wholemount, WBmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig
    Predicted to work with: Mouse, Dog, Human, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

    , corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

Target

Anti-Vesicular Acetylcholine Transporter antibody images

  • Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.

    Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
    Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
    Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
    Magnification: 40X.

References for Anti-Vesicular Acetylcholine Transporter antibody (ab31544)

ab31544 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - 0.1% Tween 20
Specification Spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mrs. Teresa Garrett

Verified customer

Submitted Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"