Overview

  • Product name
  • Description
    Rabbit polyclonal to VGF
  • Specificity
    The immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    17 amino acid peptide near the carboxy terminus of the Human VGF.

  • Positive control
    • Human Brain Cortex tissue; Human Brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • Tissue specificity
    Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • Post-translational
    modifications
    Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • Cellular localization
    Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

Images

  • ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml
    Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Human Brain tissue lysate


    Predicted band size : 67 kDa

References

ab115609 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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