The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Tissue specificityCentral and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
Post-translational modificationsMultiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Cellular localizationSecreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-VGF antibody (ab115609)
Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
Lane 1 : Human Brain tissue lysate Lane 2 : Human Brain tissue lysate
Predicted band size : 67 kDa
References for Anti-VGF antibody (ab115609)
has not yet been referenced specifically in any publications.
Publishing research using ab115609? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"