Overview

  • Product name
  • Description
    Rabbit polyclonal to VGLL3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 277-326 (CDITKTEPTT VTSATSAWAG AFHGTVDIVP SVGFDTGLQH QDKSKESPWY) of human VGLL3 (NP_057290).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-VGLL3 antibody (ab83555) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

References

ab83555 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (293 T Cell Line)
Total protein in input
500 µg
Treatment
OE of mouse VGLL3 myc tag
Immuno-precipitation step
Other - Dynabeads
Specification
293 T Cell Line
Username

Abcam user community

Verified customer

Submitted Dec 04 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (satellite cell)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
satellite cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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