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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Rat VGluT1.
(Peptide available as ab93078.)
Our Abpromise guarantee covers the use of ab77822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).|
IHC-Fr image of VGluT1 staining on zebrafish retina sections using ab77822 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab77822 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).