Overview

  • Product name
    Anti-VGLUT2 antibody [8G9.2]
    See all VGLUT2 primary antibodies
  • Description
    Mouse monoclonal [8G9.2] to VGLUT2
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Rat VGLUT2.

  • Positive control
    • Floating mouse brain tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM Sodium phosphate, pH 7.6
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    8G9.2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22064485
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
IHC-FrFl 1/50 - 1/300.

Target

  • Function
    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • Tissue specificity
    Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • Developmental stage
    Expressed in fetal brain.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Differentiation associated BNPI antibody
    • Differentiation associated Na dependent inorganic phosphate cotransporter antibody
    • Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
    • Differentiation-associated BNPI antibody
    • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
    • DNPI antibody
    • SLC17A6 antibody
    • Sodium dependent inorganic phosphate cotransporter antibody
    • Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
    • Solute carrier family 17 member 6 antibody
    • Vesicular glutamate transporter 2 antibody
    • Vesicular glutamate transporter type 2 antibody
    • VGLU2_HUMAN antibody
    • VGLUT 2 antibody
    • VGluT2 antibody
    see all

Images

  • ab79157, at 1/300 dilution, staining VGLUT2 by Immunohistochemistry using ABC with NiDAB on floating mouse brain tissue sections.
  • Immunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.

  • All lanes : Anti-VGLUT2 antibody [8G9.2] (ab79157) at 1 µg/ml

    Lane 1 : Rat Cortex Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate
    Lane 3 : Rat Substantia Nigra Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

This product has been referenced in:
  • Wang HS  et al. Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury. Neurochem Int 99:9-15 (2016). WB . Read more (PubMed: 27210824) »
  • Huo Q  et al. Prefrontal Cortical GABAergic Dysfunction Contributes to Aberrant UP-State Duration in APP Knockout Mice. Cereb Cortex N/A:N/A (2016). Read more (PubMed: 27552836) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde
Username

Dr. Hyun Jin Kim

Verified customer

Submitted Mar 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.3% in 0.1X PBS
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBSTriton 3x5mn RT
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Username

Yacine Tensaouti

Verified customer

Submitted Aug 02 2017

Application
Immunohistochemistry free floating
Sample
Zebra finch Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Sep 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Astatotilapia burtoni Tissue sections (brain)
Permeabilization
Yes - 0.1% Triton
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 02 2016

Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-10% Tris Glycine Gradient gel)
Sample
Zebra finch Tissue lysate - whole (whole brain)
Specification
whole brain
Blocking step
Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2014

Thank you for your inquiry. The above mentioned antibodies are all tested to be specific for either VGlut1 or VGlut2. The sequence homology is only 73% between the two VGlut proteins and the antibodies are all tested to be specific for either VGlut...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up