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Recombinant full length protein corresponding to Rat VGLUT2.
Our Abpromise guarantee covers the use of ab79157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22064485|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).|
|IHC-FrFl||1/50 - 1/300.|
Immunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.