Overview

  • Product name
    Anti-VGLUT3 antibody
  • Description
    Rabbit polyclonal to VGLUT3
  • Specificity
    Antiserum to rat VGLUT3 reacts specifically with 60-62 kD protein of BON cells stably expressing VGLUT3 and of total rat brain extracts. No reactivity was detected with VGLUT1 and VGLUT2.
  • Tested applications
    Suitable for: WB, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    A 16 residue synthetic peptide derived from C terminal domain of rat Vesicular Glutamate Transporter type 3 (VGLUT3).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None.
    Constituents: 50% Glycerol, Whole serum.
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Electron Microscopy
ICC/IF
  • Application notes
    Electron Microscopy: 1/5000.
    ICC/IF: 1/200.
    WB: 1/2000. Predicted molecular weight: 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
    • Tissue specificity
      Expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord and thalamus.
    • Involvement in disease
      Defects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25) [MIM:605583]. DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age-dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged.
    • Sequence similarities
      Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • deafness autosomal dominant 25 antibody
      • DFNA 25 antibody
      • DFNA25 antibody
      • SLC17A8 antibody
      • Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8 antibody
      • Solute carrier family 17 member 8 antibody
      • Vesicular glutamate transporter 3 antibody
      • VGLU3_HUMAN antibody
      • VGLUT 3 antibody
      • VGluT3 antibody
      see all

    References for Anti-VGLUT3 antibody (ab23977)

    This product has been referenced in:
    • Abazyan S  et al. Mutant disrupted-in-schizophrenia 1 in astrocytes: focus on glutamate metabolism. J Neurosci Res 92:1659-68 (2014). WB ; Mouse . Read more (PubMed: 25131692) »
    • Munguba GC  et al. Vesicular glutamate transporter 3 in age-dependent optic neuropathy. Mol Vis 17:413-9 (2011). WB ; Mouse . Read more (PubMed: 21311743) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neuron)
    Specification
    neuron
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Emily Lowry

    Verified customer

    Submitted Aug 11 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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