Overview

  • Product name
  • Description
    Rabbit polyclonal to VILIP1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human VILIP1 aa 46-75 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P62760

  • Positive control
    • 293 cell lysates, transiently transfected with the VSNL1 gene; Mouse heart tissue lysates; Human brain tissue; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 22 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Anti-VILIP1 antibody (ab170565) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 22 kDa
  • All lanes : Anti-VILIP1 antibody (ab170565) at 1/100 dilution

    Lane 1 : 293 cell lysate, non-transfected
    Lane 2 : 293 cell lysate, transiently transfected with VILIP1

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 22 kDa
  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human brain tissue, labeling VILIP1 with ab170565 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of 293 cells labeling VILIP1 with ab170565 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170565 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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